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Detection Sets
Autophagy Protein Detection Set
Background
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. In mammalian cells, APG7 is essential for autophagy conjugation systems, autophagosome formation, starvation-induced bulk degradation of proteins and organelles. Beclin-1, a coiled-coil Bcl-2-interacting protein homologous to the yeast autophagy gene apg6, is a mammalian autophagy gene that can inhibit tumorigenesis and is expressed at reduced levels in human breast carcinoma, suggesting that defects in autophagy proteins may contribute to the development or progression of tumors. PIST is a protein that interacts with Beclin-1 through its coiled-coil domain and can modulate Beclin-1 activity. LAMP1 and LAMP2 are homologous, highly glycosylated proteins associated with the lysosome and are thought to have overlapping functions. Mice lacking LAMP1 have very minor defects compared to those deficient in LAMP2 expression. However, the loss of both proteins results in embryonic lethality, suggesting that each protein possesses some unique and necessary functions.
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Description
For images please see PDF data sheet
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Kit Contents
APG7 Antibody (CT), Catalog No. 3615 (50μg)
Beclin-1 Antibody (CT), Catalog No. 3611 (50μg)
LAMP1 Antibody, Catalog No. 3629 (50μg)
LAMP2 Antibody, Catalog No. 3627 (50μg)
PIST Antibody (CT), Catalog No. 3631 (50μg)
Source
Rabbit polyclonal antibodies were raised against peptides corresponding to amino acid sequences from each of the corresponding proteins.
Application
These polyclonal antibodies can be used for detection of APG7, Beclin-1, LAMP1, LAMP2 and PIST by immunoblot at 1 – 2 μg/ml. APG7, Beclin-1 and PIST antibodies can detect their respective proteins via immunohistochemistry at 1 – 10 μg/ml.
Tested Application
WB, IHC
Blocking Peptide
APG7 peptide (CT), Catalog No. 3615P
Beclin-1 peptide (CT), Catalog No. 3611P
LAMP1 peptide, Catalog No. 3629P
LAMP2 peptide, Catalog No. 3627P
PIST peptide (CT), Catalog No. 3631P
Storage
Antibodies are supplied as affinity chromatography purified IgG, in PBS containing 0.02% sodium azide. Antibodies should be stored at -20˚C.
Positive Control
- APG7 antibody: MCF7 cell lysate, Catalog No. 1219
Beclin-1 antibody: A431 cell lysate, Catalog No. 1202
LAMP1 antibody: EL4 cell lysate, Catalog No. 1287
LAMP2 antibody: MCF7 cell lysate, Catalog No. 1219
PIST antibody: Human colon tissue lysate, Catalog No. 1320
Species Reactivity
see datasheet
Short Description
APG7 (CT), Beclin-1 (CT), LAMP1, LAMP2 and PIST (CT)
This product belongs to the following categories:
Related Products
References- Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906.
- Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.
- Komatsu M, Waguri S, Ueno T, et al. Impairment of starvation-induced and constitutively autophagy in Atg7-deficient mice. J. Cell. Biol. 2005; 169:425-34.
- Liang XH, Kleeman LK, Jiang HH, et al. Protection against fatal Sindbis virus encephalitis by Beclin, a novel Bcl-2-interacting protein. J. Virol. 1998; 72:8586-96.
Datasheet 0803-W
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