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Primary Antibodies
Aurora Kinase Antibody
Background
Aurora Kinase Antibody.
Additional Names
AURKA, aurora kinase A, AIK, ARK1, AURA, AURORA2, BTAK, MGC34538, STK15, STK6, STK7, IPL1-related kinase, aurora-A, aurora-related kinase 1, aurora/IPL1-like kinase, breast-tumor-amplified kinase, serine/threonine kinase 15, serine/threonine kinase 6, ser
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Description
Left: Western blot analysis of Aurora kinase in Jurkat lysate (RIPA buffer, 1.4E5 cells per lane). Detected by western blot using chemiluminescence using aurora kinase a antibody (0.5μg/mL).
Below: HEK293 overexpressing Aurora kinase with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel and with aurora kinase antibody in the right panel (vector-only transfection in first and fourth lanes).
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Other Product Images
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Source
Aurora kinase antibody was raised against a synthetic peptide near the C-terminus of aurora kinase.
Purification
Aurora Kinase antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
Aurora Kinase antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: antibody detection limit dilution 1:128,000. Western blot: Approx 45kDa band seen in Jurkat and HeLa cell lysates. Recommended for use at 0.5-2 μg/ml.
Tested Application
E, WB
Buffer
Aurora kinase antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-014P - Aurora Kinase Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1205 - Jurkat Cell Lysate
Species Reactivity
H
Protein Accession Number
NP_003591.2
Short Description
(CT) Aurora kinase A / STK15
This product belongs to the following categories:
Related Products
References- Zhou H, Kuang J, Zhong L, Kuo WL, Gray JW, Sahin A, Brinkley BR, Sen S. Tumour amplified kinase STK15/BTAK induces centrosome amplification, aneuploidy and transformation. Nat Genet 1998 Oct;20(2):189-93
Datasheet 09-01W
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