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Catalog Number:

45-269


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REDI-Ladder™ Molecular Weight Marker


Antibody Production


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Primary Antibodies

Apollon Antibody

Background

Apollon Antibody.

Additional Names

BIRC6, baculoviral IAP repeat-containing 6 (apollon), KIAA1289, baculoviral IAP repeat-containing 6, BRUCE, APOLLON

Source

Apollon antibody was raised against a synthetic peptide of Apollon.

Purification

Apollon antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality / Clone

This is a polyclonal antibody.

Host

Apollon antibody was raised in goat.

Please use anti-goat secondary antibodies.

Application

Peptide ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Preliminary experiments gave no signal but low background in human liver and kidney lysates at up to 1μg/ml. However this used our routine western blotting protocol which we would not expect to reliably detect proteins as large as the predicted size of approx. 555KDa according to NP_057336.

Tested Application

E

Buffer

Apollon antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Blocking Peptide

Cat. No. 45-269P - Apollon Peptide

Storage

Aliquot and store at -20°C. Minimize freezing and thawing.

Species Reactivity

H

Protein Accession Number

NP_057336

Short Description

BIRC6 / Apollon

This product belongs to the following categories:

Related Products

References

  1. Chen Z, Naito M, Hori S, Mashima T, Yamori T, Tsuruo T. A human IAP-family gene, apollon, expressed in human brain cancer cells. Biochem Biophys Res Commun. 1999 Nov 2;264(3):847-54.


Datasheet 08-01W

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