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Primary Antibodies
Apolipoprotein B Antibody
Background
Apolipoprotein B Antibody.
Additional Names
APOB, apolipoprotein B (including Ag(x) antigen), FLDB, apoB-100, apoB-48, apolipoprotein B, apolipoprotein B48
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Description
Left: Western blot analysis of Apolipoprotein B in human serum lysate using Apolipoprotein B antibody (0.5μg/ml). Data kindly provided by Yan Xie and NO Davidson, Washington USA. This antibody showed background below 150kDa in serum samples.
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Source
Apolipoprotein B antibody was raised against a synthetic peptide of Apolipoprotein B.
Purification
Apolipoprotein B antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
Apolipoprotein B antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Bands corresponding to the exopected sizes of ApoB100 and ApoB48 were detected in Human serum samples from five different individuals. Recommended concentration: 0.3-1μg/ml.
Tested Application
E, WB
Buffer
Apolipoprotein B antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 46-802P - Apolipoprotein B Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Species Reactivity
H
Protein Accession Number
NP_000375.2
Short Description
APOB
This product belongs to the following categories:
Related Products
References- Benn M, Nordestgaard BG, Jensen JS, Tybjaerg-Hansen A. Polymorphisms in apolipoprotein B and risk of ischemic stroke. J Clin Endocrinol Metab. 2007 Sep;92(9):3611-7
Datasheet 09-01W
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