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Catalog Number:

46-747


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REDI-Ladder™ Molecular Weight Marker


Antibody Production


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Primary Antibodies

ApoL5 Antibody

Background

ApoL5 Antibody.

Additional Names

APOL5, apolipoprotein L, 5, APOL-V, APOLV, OTTHUMP00000028773, apolipoprotein L-V, apolipoprotein L

ApoL5 Antibody

Description

Left: Western blot analysis of ApoL5 in human frontal lobe lysate (35μg protein in RIPA buffer) using ApoL5 antibody (1μg/mL).

Source

ApoL5 antibody was raised against a synthetic peptide near the C-Terminus of ApoL5.

Purification

ApoL5 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality / Clone

This is a polyclonal antibody.

Host

ApoL5 antibody was raised in goat.

Please use anti-goat secondary antibodies.

Application

Peptide ELISA: Antibody detection limit dilution 1:8,000. Western Blot: Approx. 48kDa band observed in human brain (frontal cortex) lysates (calculated MW of 47.0kDa according to NP_085145.1). Recommended concentration: 1-3μg/ml.

Tested Application

E, WB

Buffer

ApoL5 antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Blocking Peptide

Cat. No. 46-747P - ApoL5 Peptide

Storage

Aliquot and store at -20°C. Minimize freezing and thawing.

Positive Control

  1. Cat. No. 1366 - Human Frontal Lobe Tissue Lysate

Species Reactivity

H

Protein Accession Number

NP_085145.1

Short Description

APOL5

This product belongs to the following categories:

Related Products

References

  1. Page NM, Butlin DJ, Lomthaisong K, Lowry PJ. The human apolipoprotein L gene cluster: identification, classification, and sites of distribution. Genomics. 2001 May 15;74(1):71-8.


Datasheet 09-01W

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