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Primary Antibodies
ApoL1 Antibody
Background
ApoL1 Antibody. This antibody is expected to recognize both reported isoforms (NP_003652.2; NP_663318.1).
Additional Names
APOL1, apolipoprotein L, 1, APO-L, APOL, APOL-I, OTTHUMP00000028705, apolipoprotein L-I, apolipoprotein L1
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Description
Left: Western blot analysis of ApoL1 in human frontal lobe lysate (35μg protein in RIPA buffer) using ApoL1 antibody (1μg/mL).
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Source
ApoL1 antibody was raised against a synthetic peptide near the C-Terminus of ApoL1.
Purification
ApoL1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
ApoL1 antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: Antibody detection limit dilution 1:8,000. Western Blot: Approx. 45kDa band observed in human brain (frontal cortex) lysates (calculated MW of 44.0kDa according to NP_003652.2;). Recommended concentration: 1-3μg/ml.
Tested Application
E, WB
Buffer
ApoL1 antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 46-746P - ApoL1 Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1366 - Human Frontal Lobe Tissue Lysate
Species Reactivity
H
Protein Accession Number
NP_003652.2, NP_663318.1
Short Description
APOL1
This product belongs to the following categories:
Related Products
References- Pérez-Morga D, Vanhollebeke B, Paturiaux-Hanocq F, Nolan DP, Lins L, Homblé F, Vanhamme L, Tebabi P, Pays A, Poelvoorde P, Jacquet A, Brasseur R, Pays E. Apolipoprotein L-I promotes trypanosome lysis by forming pores in lysosomal membranes. Science. 2005 Jul 15;309(5733):469-72.
Datasheet 09-01W
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