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Recombinant Proteins

Apo-SAA1 Recombinant Protein

Background

Serum amyloid A proteins (SAA) represents a family of apolipoproteins that circulates in association with high-density lipoproteins (HDL). The level of apo-SAA, normally 1-5 μg/ml in plasma, increases 500-1000 fold within 24 hours of an inflammatory stimulus and, under these conditions, is the most abundant HDL apo-lipoprotein. The human SAA gene codes for a 122 amino acid nonglycosylated polypeptide, which contains an 18 amino acid N-terminal sequence. Recombinant human apo-SAA1 is an 11.7 kDa protein containing 105 amino acid residues.

Additional Names

apo-SAA, apo-Serum amyloid A 1

Location

MRSFFSFLGE AFDGARDMWR AYSDMREANY IGSDKYFHAR GNYDAAKRGP GGVWAAEAIS DARENIQRFF GHGAEDSLAD QAANEWGRSG KDPNHFRPAG LPEKY

Source

E.coli

Reactivity

n/a

Preparation

Determined by its ability to chemoattract human monocytes using a concentration range of 10.0 - 10.0 ng/ml.

Tested Application

n/a

Storage

The lyophilized Apo-SAA1 recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted Apo-SAA1 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing Apo-SAA1 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

Species Reactivity

n/a

Purity

Greater than 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin level is less than 0.1 ng per μg (1EU/μg).

Short Description

Recombinant Human Apo-SAA1

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