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Primary Antibodies
Apaf1 Antibody
Background
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1) (1,2). Apaf1 binds to cytochrome c (Apaf-2) and caspase-9 (Apaf-3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (3). Apaf1 can also associate with caspase-4 and caspase-8 (4). Apaf1 is ubiquitously expressed in human tissues .
Additional Names
Apaf1 (NT), Apaf1, Apaf-1
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Description
Left: Western blot analysis of Apaf1 in K562 cell lysate with Apaf1 antibody at 1 μg/ml in the (A) absence and (B) presence of blocking peptide.
Below: Immunohistochemistry of Apaf1 in human heart tissue with Apaf1 antibody at 1 μg/ml.
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Other Product Images
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Source
Apaf1 antibody was raised against a peptide corresponding to amino acids near the amino terminus of human Apaf1. The sequences of the immunogenic peptide are identical between human and mouse.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
Apaf1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human Apaf1 (N-Terminus) Peptide (Cat. No. 2013P)
Application
Apaf1 antibody can be used for detection of Apaf1 by Western blot at 1 – 2 μg/ml. (Optimal dilution should be determined by user.) Whole cell lysate from HeLa cells can be used as positive control and a 115 - 130 kDa band should be detected.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2013P - Apaf1 Peptide
Storage
Apaf1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1204 - K562 Cell Lysate
Species Reactivity
H, M, R
Protein GI Number
2330015
Protein Accession Number
AAC51678
Short Description
(NT) Apoptotic protease activating factor 1
This product belongs to the following categories:
Related Products
References- Zou H, Henzel WJ, Liu X, Lutschg A, Wang X. Apaf1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell 1997;90:405-13
- Cecconi F, Alvarez-Bolado G, Meyer BI, Roth KA, Gruss P. Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development. Cell 1998;94:727-37
- Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES, Wang X. Cytochrome c and dATP-dependent formation ofApaf1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997;91:479-89
- Hu Y, Benedict MA, Wu D, Inohara N, Nunez G. Bcl-XL interacts with Apaf1 and inhibits Apaf1-dependent caspase-9 activation. Proc Natl Acad Sci USA 1998;95:4386-91 (RD1299)
Datasheet 08-02W
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