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Primary Antibodies
Apaf-1 Monoclonal Antibody
Background
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologous of the key cell death gene CED-4 in C. elegans was identified recently from human and mouse and designated Apaf1 for apoptosis protease-activating factor 1 (1,2). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (3). Apaf1 can also associate with caspase-4 and caspase-8 (4). Apaf1 transcript is ubiquitously expressed in human tissues (1).
Additional Names
Apaf-1 (5E11), Apoptotic protease activating factor 1
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Description
Immunohistochemistry of Apaf1 in K562 cells with Apaf1 antibody at 0.5 µg/ml.
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Source
Mouse monoclonal Apaf1 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human Apaf1 (1)
Purification
Immunoaffinity chromotography purified IgG
Clonality / Clone
This is a monoclonal antibody. (Clone 5E11)
Host
Apaf-1 monoclonal antibody was raised in mouse.
Please use anti-mouse secondary antibodies.
Immunogen
A peptide corresponding to amino acids near the carboxy terminus of human Apaf1.
Application
Apaf1 antibody can be used for detection of Apaf1 by immunohistochemistry at 5 – 10 mg/ml. Apaf1 has not been confirmed for use in immunoblots.
Tested Application
E, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Storage
Apaf-1 monoclonal antibody can be stored at 4˚C, stable for one year.
Positive Control
- Cat. No. 1204 - K562 Whole Cell Lysate
Species Reactivity
H, M, R
Short Description
Apoptosis protease activator
This product belongs to the following categories:
Related Products
References- Zou H, Henzel WJ, Liu X, et al. Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3. Cell 1997; 90:405-13.
- Cecconi F, Alvarez-Bolado G, Meyer BI, et al. Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development. Cell 1998; 94:727-37.
- Li P, Nijhawan D, Budihardjo I, et al. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997; 91:479-89.
- Hu Y, Benedict MA, Wu D, et al. Bcl-XL interacts with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation. Proc. Natl. Acad. Sci. USA 1998; 95:4386-91.
Datasheet 0803-W
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