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Primary Antibodies
Annexin Antibody
Background
Annexin Antibody. This antibody is expected to recognize both reported isoforms. NP_004030.1 and NP_001002857.1 are varients of isoform 2.
Additional Names
ANXA2, annexin A2, ANX2, LIP2, LPC2, CAL1H, LPC2D, ANX2L4, P36, PAP-IV, annexin II, lipocortin II, Annexin II (lipocortin I), annexin II (lipocortin II), calpactin I, heavy polypeptide (p36), annexin II (lipocortin II calpactin I, heavy polypeptide), calp
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Description
Left: Western blot analysis of Annexin in A431 lysate (RIPA buffer, 35μg total protein per lane) using Annexin A2 antibody (1μg/mL).
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Source
Annexin antibody was raised against a synthetic peptide near the N-terminus of Annexin A2.
Purification
Annexin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
Annexin antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: Antibody detection limit dilution 1:4,000. Western Blot: Approx 35kDa band observed in lysates of cell lines A431 and A549 (calculated MW of 38.6kDa according to NP_004030.1 and NP_001002857.1). Recommended concentration: 1-3μg/ml.
Tested Application
E, WB
Buffer
Annexin antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-008P - Annexin Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1202 - A431 Cell Lysate
Species Reactivity
H, M, R, B, P
Protein Accession Number
NP_001002858.1, NP_004030.1, NP_001002857.1
Short Description
(NT) Annexin A2
This product belongs to the following categories:
Related Products
References- Takahashi S, Reddy SV, Chirgwin JM, Devlin R, Haipek C, Anderson J, Roodman GD. Cloning and identification of annexin II as an autocrine/paracrine factor that increases osteoclast formation and bone resorption. J Biol Chem 1994 Nov 18;269(46):28696-701
Datasheet 08-02W
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