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Primary Antibodies
Annexin A10 Antibody
Background
Annexin A10 Antibody.
Additional Names
ANXA10, annexin A10, ANX14, annexin 14
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Description
Left: Immunohistochemistry staining of Annexin A10 in paraffin embedded human pancreas using Annexin A10 antibody at 3.8μg/mL.
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Source
Annexin A10 antibody was raised against a synthetic peptide of Annexin A10.
Purification
Annexin A10 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
Annexin A10 antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: antibody detection limit dilution 1:8000. Western Blot: Preliminary experiments (at 1μg/ml) gave an approx 45-48kDa band in Hela, A431 and Human Kidney lysates. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 37kDa according to NP_009124. Immunohistochemistry: In paraffin embedded Human Pancreas shows staining of islet of Langerhans . Recommended concentration, 3-5μg/ml.
Tested Application
E, IHC
Buffer
Annexin A10 antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-255P - Annexin A10 Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Species Reactivity
H, M
Protein Accession Number
NP_009124
Short Description
Annexin A10 / Annexin 14
This product belongs to the following categories:
Related Products
References- Morgan RO, Jenkins NA, Gilbert DJ, Copeland NG, Balsara BR, Testa JR, Fernandez MP. Novel human and mouse annexin A10 are linked to the genome duplications during early chordate evolution. Genomics. 1999 Aug 15;60(1):40-9.
Datasheet 08-01W
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