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Primary Antibodies
Aminopeptidase A Antibody
Background
Aminopeptidase A Antibody.
Additional Names
ENPEP, glutamyl aminopeptidase (aminopeptidase A), HGNC:3355, APA, gp160, aminopeptidase A
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Description
Left: Western blot analysis of Aminopeptidase A in human testis lysate (RIPA buffer, 30μg total protein per lane) using Aminopeptidase A antibody (1μg/mL).
Below: Immunohistochemistry staining of Aminopeptidase A in paraffin embedded human kidney using Aminopeptidase A antibody at 3.8μg/mL.
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Other Product Images
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Source
Aminopeptidase A antibody was raised against a synthetic peptide of Aminopeptidase A.
Purification
Aminopeptidase A antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
Aminopeptidase A antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: antibody detection limit dilution 1:64000. Western Blot: Approx 170kDa band observed in human brain and testis lysates (calculated MW of 109kDa according to NP_001968.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Ando et al, J Clin Endocrinol Metab. 2002 May;87(5):2303-9; PMID: 11994381). Recommended concentration: 1-3μg/ml. Immunohistochemistry: In paraffin embedded Human Kidney shows staining of basement membrane and the mesangium in the glomerulus. Recommended concentration, 3-5μg/ml.
Tested Application
E, WB, IHC
Buffer
Aminopeptidase A antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-249P - Aminopeptidase A Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1313 - Human Testis Tissue Lysate
Species Reactivity
H, M, R, D, P
Protein Accession Number
NP_001968.2
Short Description
Aminopeptidase A
This product belongs to the following categories:
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References- Malerba G, Pignatti PF. A review of asthma genetics: gene expression studies and recent candidates. J Appl Genet. 2005;46(1):93-104. Review.
Datasheet 08-01W
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