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Detection Sets
Alzheimer’s Disease gamma-Secretase Detection Set
Background
Accumulation of the amyloid-β peptide (Aβ) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer’s disease. The β-amyloid protein precursor (APP) is cleaved by three enzymes (TACE, BACE/BACE2 and γ-secretase) at three distinct sites (α, β and γ respectively). The γ-secretase complex is a membrane-bound aspartyl protease that can cleave certain proteins at peptide bonds buried within the hydrophobic environment of the lipid bilayer and is composed of the proteins APH1, nicastrin, PEN2 and presenilin1. Its cleavage of APP results in either the non-toxic p3 (from the α and γ cleavage site) or the toxic Aβ (from the β and γ cleavage site). APH1 was initially identified as a component of the Notch pathway and exists in at least three distinct isoforms with APH1a as the principal isoform present in the γ-secretase complex. Mice deficient in this isoform, but not the other two, were lethal at E10.5, with impaired vascular and neural development observed. Besides acting as a critical component of the γ-secretase complex, nicastrin is also thought to be involved in cell proliferation and signaling, especially in regards to activation of Notch receptors as loss of nicastrin expression results in mouse embryonic lethality. Presenilin1 was initially identified a marker of susceptibility to early-onset Alzheimer’s disease.
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Description
For images please see PDF data sheet
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Kit Contents
APH1 Antibody (NT), Catalog No. 4001 (50μg)
Nicastrin Antibody (CT), Catalog No. 3983 (50μg)
PEN2 Antibody (CT), Catalog No. 3979 (50μg)
Presenilin1 Antibody, Catalog No. 4203 (50μg)
Source
Rabbit polyclonal antibodies were raised against peptides corresponding to amino acid sequences from each of the corresponding proteins.
Application
These polyclonal antibodies can be used for detection of APH1, nicastrin, PEN2 and presenilin1 by immunoblot at 0.5 – 4 μg/ml
Tested Application
WB
Blocking Peptide
APH1 Peptide (NT), Catalog No. 4001P
Nicastrin Peptide (CT), Catalog No. 3983P
PEN2 Peptide (CT), Catalog No. 3979P
Presenilin1 Peptide, Catalog No. 4203P
Storage
Antibodies are supplied as affinity chromatography purified IgG, in PBS containing 0.02% sodium azide. Antibodies should be stored at -20˚C.
Positive Control
- APH1 Antibody: RAW264.7 Cell Lysate, Catalog No. 1223
Nicastrin Antibody: Mouse Brain Tissue Lysate, Catalog No. 1403
PEN2 Antibody: K562 Cell Lysate, Catalog No. 1204
Presenilin1 Antibody: Human Brain Tissue Lysate, Catalog No. 1303
Species Reactivity
see datasheet
Short Description
APH1 (NT), Nicastrin (CT), PEN2 (CT), and Presenilin1
This product belongs to the following categories:
Related Products
References- Vassar R, Bennett BD, Babu-Khan S, et al. Beta-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE. Science 1999; 286:735-41.
- Sinha S, Anderson JP, Barbour R, et al. Purification and cloning of amyloid precursor protein beta-secretase from human brain. Nature 1999; 402:537-40.
- Selkoe DJ. The cell biology of b-amyloid precursor protein and presenilin in Alzheimer’s disease. Trends Cell Biol. 1998; 8:447-53.
- Goutte C, Tsunozaki M, Hale VA, et al. APH-1 is a multipass membrane protein essential for the Notch signaling pathway in Caenorhabditis elegans embryos. Proc. Natl. Acad. Sci. USA 2002; 99:775-9.
Datasheet 0803-W
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