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Detection Sets
Alzheimer’s Disease B-Amyloid Protein Detection Set
Background
Accumulation of the amyloid-β peptide (Aβ) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer’s disease. The βamyloid protein precursor (APP) is cleaved by one of two βsecretases (BACE and BACE2), producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for? βsecretase to generate the 4 kDa amyloid-β peptide (Aβ), which is deposited in the Alzheimer’s disease patients’ brains. BACE was identified by several groups independently and designated β-site APP cleaving enzyme (BACE) . BACE is a transmembrane aspartic protease and co-localizes with APP. BACE2 also cleaves APP at β-site and at a different site within Aβ. BACE2 locates on chromosome 21q22.3, the so-called ‘Down critical region’, suggesting that BACE2 and Aβ may also contribute to the pathogenesis of Down syndrome.
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Description
For images please see PDF data sheet
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Kit Contents
APP Antibody (CT), Catalog No. 2136 (50μg)
APP Antibody (Aβ-NT), Catalog No. 2133 (50μg)
BACE Antibody (CT), Catalog No. 2253 (50μg)
BACE2 Antibody (NT), Catalog No. 2247 (50μg)
Source
Rabbit polyclonal antibodies were raised against peptides corresponding to amino acid sequences from each of the corresponding proteins. Both APP antibodies will react with the C99 fragment of APP.
Application
These polyclonal antibodies can be used for detection of APP, BACE and BACE2 by immunoblot at 1 – 5 µg/ml. APP and BACE antibodies can detect their respective proteins via immunohistochemistry at 2 – 10 μg/ml. Human brain lysate and tissue sections can be used as a positive control for each of these antibodies
Tested Application
WB, IHC
Storage
Antibodies are supplied as affinity chromatography purified IgG, in PBS containing 0.02% sodium azide. Antibodies should be stored at -20˚C.
Species Reactivity
see datasheet
Short Description
APP (CT), APP (Aβ-NT), BACE and BACE2
This product belongs to the following categories:
Related Products
References- Vassar R, Bennett BD, Babu-Khan S, et al. Beta-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE. Science 1999; 286:735-41.
- Sinha S, Anderson JP, Barbour R, et al. Purification and cloning of amyloid precursor protein beta-secretase from human brain. Nature 1999; 402:537-40.
- Acquati F, et al. The gene encoding DRAP (BACE2), a glycosylated transmembrane protein of the aspartic protease family, maps to the down critical region. FEBS Lett 2000;468:59-64
- Solans A, et al. A new aspartyl protease on 21q22.3, BACE2, is highly similar to Alzheimer's amyloid precursor protein beta-secretase. Cytogenet Cell Genet 2000;89:177-184.
Datasheet 0803-W
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