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Primary Antibodies
Alpha2-antiplasmin Antibody
Background
Alpha2-antiplasmin Antibody.
Additional Names
SERPINF2, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2, A2AP, AAP, ALPHA-2-PI, API, PLI, alpha-2-antiplasmin, alpha-2-plasmin inhibitor, serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
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Description
Left: Western blot analysis of Alpha2-antiplasmin in human duodenum lysate (35μg protein in RIPA buffer) using Alpha2-antiplasmin antibody (0.1μg/mL).
Below: Immunohistochemistry staining of Alpha2-antiplasmin in paraffin embedded human liver using Alpha2-antiplasmin antibody at 3.8μg/mL.
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Other Product Images
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Source
Alpha2-antiplasmin antibody was raised against a synthetic peptide of Alpha2-antiplasmin.
Purification
Alpha2-antiplasmin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
Alpha2-antiplasmin antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: antibody detection limit dilution 1:16000. Western Blot: Approx. 70kDa band observed in Human Duodenum and Ileum lysates (calculated MW of 54.6kDa according to NP_000925.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Lee et al, Blood. 2004 May 15;103(10):3783-8.; PMID: 14751930). Recommended concentration: 0.3-1μg/ml. An additional band of unknown identity was also consistently observed at 26kDa. This band was successfully blocked by incubation with the immunising peptide. Immunohistochemistry: In paraffin embedded Human Liver shows potty cytoplasm staining in hepatocytes. Recommended concentration, 3-5μg/ml.
Tested Application
E, WB, IHC
Buffer
Alpha2-antiplasmin antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-243P - Alpha2-antiplasmin Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1364 - Human Duodenum Tissue Lysate
Species Reactivity
H
Protein Accession Number
NP_000925.2
Short Description
Alpha2-antiplasmin
This product belongs to the following categories:
Related Products
References- Matsuno H, Okada K, Ueshima S, Matsuo O, Kozawa O. Alpha2-antiplasmin plays a significant role in acute pulmonary embolism. J Thromb Haemost. 2003 Aug;1(8):1734-9
Datasheet 08-01W
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