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Primary Antibodies
Alpha-tubulin Antibody
Background
Alpha-tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Another post-translational modification of detyrosinated alpha-tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. Like GAPDH and beta-Actin, this antibody makes an excellent loading control in immunoblots.
Additional Names
Alpha-tubulin, Tubulin alpha-1A, TUBA1A, TUBA3, LIS3
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Description
Left: Western blot analysis of Tubulin in rat brain tissue lysate with Tubulin antibody at (A) 0.5 and (B) 1 μg/ml.
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Source
Tubulin antibody was raised against a 16 amino acid peptide near the amino terminus of human Tubulin.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
Alpha-tubulin antibody was raised in chicken.
Please use anti-chicken secondary antibodies.
Application
Tubulin antibody can be used for detection of Tubulin by Western blot at 1 - 2 μg/ml.
Tested Application
E, WB
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 5103P - Alpha-tubulin Peptide
Storage
Alpha-tubulin antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1463 - Rat Brain Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
37492
Protein Accession Number
CAA25855
Short Description
Tubulin alpha-1A
This product belongs to the following categories:
References- McKean PG, Vaughan S, and Gull K. The extended tubulin family. J. Cell Sci. 2001; 114:2723-33.
- Barra HA, Arce CA, and Argarana CE. Posttranslational tyrosination/detyrosination of tubulin. Mol. Neurobiol. 1988; 2:133-53.
- Fukshima N, Furuta D, Hidaka Y, et al. Post-translational modifcations of tubulin in the nervous system. J. Neurochem. 2009; 109:683-693.
Datasheet 09-01W
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