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Secondary Antibodies
Alkaline Phosphatase Conjugated Affinity Purified anti-Chicken IgY [H&L] [Rabbit]
Background
Alkaline Phosphatase Conjugated Affinity Purified anti-Chicken IgY [H&L] [Rabbit]
Conjugate
Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
Purification
Alkaline Phosphatase Conjugated Affinity Purified anti-Chicken IgY was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgY coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Chicken IgY and Chicken Serum.
Host
Rabbit
Immunogen
Chicken IgY whole molecule
Preparation
PHYSICAL STATE: Liquid (sterile filtered)
ANTIBODY CONCENTRATION: 1.0 mg/ml (by UV absorbance at 280 nm)
Application
Alkaline Phosphatase Conjugated Affinity Purified anti-Chicken IgY is suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 mg of Chicken IgY in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:12,000 is suggested for this product. (Optimal dilution should be determined by user).
Tested Application
E
Buffer
0.05M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Storage
Alkaline Phosphatase Conjugated Affinity Purified anti-Chicken IgY can be stored vial at 4˚C before opening. DO NOT FREEZE. This product is stable at 4˚C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening product.
Species Reactivity
C
Formulation
STABILIZER:10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free
PRESERVATIVE: 0.01% (w/v) Sodium Azide
Short Description
Alkaline Phosphatase Conjugated Affinity Purified anti-Chicken IgY
This product belongs to the following categories:
References- Modified from Avarameas and Ternyrock, Immunochemistry 32; 1175 1971.
Datasheet 09-01W
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