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Primary Antibodies
Aldh3A1 Antibody
Background
Aldh3A1 is a member of the aldehyde dehydrogenase superfamily, a group of NAD(P)(+)-dependent enzymes that catalyze the oxidation of a wide spectrum of aliphatic and aromatic aldehydes. Aldh3A1 is highly expressed in stomach and even more strongly in cornea, representing between 5 to 50% of the water soluble protein fraction in mammalian corneas. It is thought that Aldh3A1 acts to protect the cornea from UV-induced oxidative stress by not only detoxification of reactive aldehydes by also through the direct absorbtion of UV energy. However, corneas from Aldh3A1-null mice are indistinguishable from those from wild-type mice; mice lacking both Aldh3A1 and Aldh1A1 showed increased cataract formation following UVB exposure, suggesting that Aldh1A1 may be able to compensate for the loss of Aldh3A1. At least two isoforms of Aldh3A1 are known to exist. This antibody is predicted to have no cross-reactivity to Aldh3A2.
Additional Names
Aldh3A1,Aldehyde dehydrogenase family3 member A1, AldhIII
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Description
Left: Western blot analysis of Aldh3A1 in human stomach lysate with Aldh3A1 antibody at (A) 1 and (B) 2 μg/ml.
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Source
Aldh3A1 antibody was raised against a 13 amino acid peptide near the carboxy terminus of the human Aldh3A1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
Aldh3A1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
Aldh3A1 antibody can be used for detection of Aldh3A1 by Western blot at 1 – 2 μg/ml.
Tested Application
E, WB
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4787P - Aldh3A1 Peptide
Storage
Aldh3A1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1319 - Human Stomach Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
22907049
Protein Accession Number
NP_000682
Short Description
Aldehyde dehydrogenase family3 member A1
This product belongs to the following categories:
Related Products
References- Vasiliou V and Pappa A. Polymorphisms of human aldehyde dehydrogenases. Consequences for drug metabolism and disease. Pharmacology 2000; 61:192-8.
- Hsu LC, Chang WC, Shibuya A, et al. Human stomach aldehyde dehydrogenase cDNA and genomic cloning, primary structure, and expression in Escheria coli. J. Biol. Chem. 1992; 267:3030-7.
- Pappa A, Sophos NA and Vasiliou V. Corneal and Stomach expression of aldehyde dehydrogenases: from fish to mammals. Chem. Biol. Interact. 2001; 130:181-91.
- Estey T, Cantore M, Weston PA, et al. Mechanisms involved in the protection of UV-induced protein inactivation by the corneal crystallin ALDH3A1. J. Biol. Chem. 2007; 282:4382-92.
Datasheet 08-01W
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