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Primary Antibodies
Akt3 Monoclonal Antibody
Background
Akt3 (also designated protein kinase B gamma or v-akt murine thymoma viral oncogene homolog 3)?with 479-amino acid protein (about 53kDa), belongs to the AKT serine/threonine protein kinase family, which also includes Akt1 and Akt2. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake.Akt3 is not required for the maintenance of normal carbohydrate metabolism but is essential for the attainment of normal organ size. Identifying Akt3 as a selective target in melanoma cells also provides new therapeutic opportunities for patients in the advanced stages of this disease.
Additional Names
Akt3
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Description
Western blot analysis using Akt3 monoclonal antibody against truncated Akt3 recombinant protein and human ovary carcinoma tissue lysate .
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Clonality / Clone
This is a monoclonal antibody. Clone 3B12F3F10, 2D2B11B11, 2D2B11H5.
Host
Akt3 monoclonal antibody was raised in mouse.
Please use anti-mouse secondary antibodies.
Immunogen
Ni-NTA purified truncated recombinant Akt3 expressed in E. Coli strain BL21 (DE3).
Application
Western Blot:1:500 - 1:2000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Tested Application
E, WB
Storage
Akt3 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Species Reactivity
H
Formulation
Ascitic fluid containing 0.03% sodium azide.
Purified antibody available at no extra cost.
Protein GI Number
10000
Short Description
also designated protein kinase B gamma or v-akt murine thymoma viral oncogene homolog 3
This product belongs to the following categories:
Related Products
References- Rachael M. Easton, Han Cho, Kristin Roovers. Mol. Cell. Biol., Mar 2005; 25: 1869 – 1878.
- Jill M. Stahl, Arati Sharma, Mitchell Cheung. Cancer Res., Oct 2004; 64: 7002–7010.
Datasheet 0903-W
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