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Primary Antibodies
Akt2 Monoclonal Antibody
Background
Akt2 (also designated protein kinase B beta or v-akt murine thymoma viral oncogene homolog 2 ), with 481-amino acid protein (about 53kDa), belongs to the AKT serine/threonine protein kinase family, which also includes Akt1 and Akt3.They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. Among the members of AKT family, Akt2 is associated with the development of human cancers. Akt2 inhibits cisplatin-induced JNK/p38 and Bax activation through phosphorylation of ASK1 and thus, plays an important role in chemoresistance.Further, Akt2 plays a specific role in muscle differentiation.
Additional Names
PKBB, PRKBB, PKBBETA, RAC-BETA
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Description
Western blot analysis using Akt2 monoclonal antibody against truncated Akt2 recombinant protein and A431 cell lysate.
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Clonality / Clone
This is a monoclonal antibody. Clone 1G8C12.
Isotype
IgG2b
Host
Akt2 monoclonal antibody was raised in mouse.
Please use anti-mouse secondary antibodies.
Immunogen
Ni-NTA purified truncated recombinant Akt2 expressed in E. Coli strain BL21 (DE3).
Application
Western Blot:1:100 - 1:500
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Tested Application
E, WB
Storage
Akt2 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1202 - A431 Cell Lysate
Species Reactivity
H
Formulation
Ascitic fluid containing 0.03% sodium azide.
Purified antibody available at no extra cost.
Protein GI Number
208
Short Description
also designated protein kinase B beta or v-akt murine thymoma viral oncogene homolog 2
This product belongs to the following categories:
Related Products
References- Zeng-qiang Yuan , Richard I. Feldman, Gene E. Sussman. J. Biol. Chem., Jun 2003; 278: 23432-23440.
- Satoshi Kaneko, Richard I. Feldman, Lu Yu. J. Biol. Chem., Jun 2002; 277: 23230-23235.
Datasheet 0903-W
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