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Recombinant Proteins
Adiponectin Recombinant Protein
Background
Adiponectin is an adipose-derived secreted protein containing 226 amino acid residues. It is relatively abundant in humans and rodents, accounting for about 0.01% of total plasma protein. The circulating levels of adiponectin are decreased under conditions of obesity, insulin resistance, and type II diabetes. Disruption of adiponectin in mice causes insulin resistance and neointimal formation. Conversely, administration of recombinant adiponectin suppresses hepatic glucose production, and reverses insulin resistance associated with both lipoatrophy and obesity. The protective role of adiponectin is attributed to its anti-inflammatory properties (e.g. ability to suppress expression of TNF-α and class A scavenger receptor in macrophages). Recombinant adiponectin is a multimeric glycoprotein containing amino acids Glu-19 to Asn-244 of the adiponectin precursor protein fused to an N-terminal histidine tag. Monomeric glycosylated adiponectin migrates at an apparent molecular weight of approximately 35.0 kDa by SDS PAGE analysis under reducing conditions.
Additional Names
Acrp-30, GBP-28, Apm-1
Location
RGHHHHHHHH ETTTQGPGVL LPLPKGACTG WMAGIPGHPG HNGAPGRDGR DGTPGEKGEK GDPGLIGPKG DIGETGVPGA EGPRGFPGIQ GRKGEPGEGA YVYRSAFSVG LETYVTIPNM PIRFTKIFYN QQNHYDGSTG KFYCNIPGLY YFSYHITVYM KDVKVSLFKK DKAVLFTYDQ YQEKNVDQAS GSVLLHLEVG DQVWLQVYGD GDHNGLYADN VNDSTFTGFL LYHDTN
Source
Hi-5 insect cells
Reactivity
n/a
Preparation
Biological activity was determined by a cytotoxic assay using M1 cells. The ED50 for this effect is 3.0 - 6.0 ?g/ml.
Tested Application
n/a
Storage
The lyophilized Adiponectin recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted Adiponectin is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing Adiponectin recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Species Reactivity
n/a
Purity
Greater than 97% by SDS-PAGE gel and HPLC analyses.
Endotoxin level is less than 0.1 ng per μg (1EU/μg).
Short Description
Recombinant Human Adiponectin
This product belongs to the following categories:
Datasheet 0904-W
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