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XP-5102


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REDI-Ladder™ Molecular Weight Marker


Antibody Production


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Primary Antibodies

Adipolean Variant Antibody

Background

Adipolean Variant Antibody

Source

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hAdipolean Variant (human Adipolean Variant).

Clonality / Clone

This is a polyclonal antibody.

Host

Adipolean Variant antibody was raised in rabbit.

Please use rabbit secondary antibodies.

Preparation

A sterile filtered antibody solution (1 mg/mL) was lyophilized from 0.5X PBS pH 7.2.

Application

ELISA:

To detect hAdipolean Variant by direct ELISA (using 100 μl/well antibody solution) a concentration of at least 0.5μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hAdipolean Variant.

Western Blot:

To detect hAdipolean Variant by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hAdipolean Variant is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Tested Application

E, WB

Storage

Adipolean Variant lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/ml. The antibody is stable for at least six weeks at 2-4˚C when reconstituted.

Species Reactivity

H

Short Description

Anti-Human Adipolean Variant

This product belongs to the following categories:

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Datasheet 1001-W

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