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Primary Antibodies
Adipolean Antibody
Background
Adipolean Biotin Conjugated Antibody
Additional Names
ACDC, ACRP30, Adipocyte C1q and collagen domain containing protein, Adipocyte complement related protein, ADIPOQ, Adipose most abundant gene transcript 1, GBP28, Gelatin binding protein
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Description
Adipose cells produce and secrete numerous physiologically important proteins, such as Lipoprotein Lipase, Leptin, and Adipocyte Complement Related protein of 30 kDa, also known as Acrp30 or Adiponectin. Adiponectin is a circulating protein that is secreted exclusively by differentiated adipocytes. During adipocyte differentiation, Adiponectin mRNA is induced >100 fold. Adiponectin improves the ability of insulin to suppress glucose production, at sub physiological levels, thereby linking adipose tissue to whole body glucose regulation. Adiponectin function appears to be regulated by phosphatidylinositol 3 kinase (PI3K) since Adiponectin secretion is blocked by pharmacologic inhibitors of this kinase. Adiponectin mRNA is significantly reduced in adipose tissue of obese patients with Type 2 diabetes. The structural similarity of Adiponectin to TNF alpha suggests that Adiponectin may play a role in pathogenesis of insulin resistance in Type 2 diabetes. Adiponectin is implicated as a regulator of whole body energy homeostasis.
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Source
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant (human Adipolean).
Conjugate
Biotin
Clonality / Clone
This is a polyclonal antibody.
Host
Adipolean antibody was raised in goat.
Please use goat secondary antibodies.
Preparation
Lyophilized from sterile-filtered PBS solution.
Application
ELISA:
To detect hAdipolean by direct ELISA (using 100 μl/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μg/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hAdipolean.
Western Blot:
To detect hAdipolean by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hAdipolean is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Tested Application
E, WB
Storage
Adipolean lyophilized antibody is stable for at least 6 months when kept at -20˚C. The biotinylated antibody should be reconstituted to a concentration of 0.1 - 1.0 mg/ml with sterile PBS solution containing 0.1% BSA. This solution can be stored at 4˚C for at least one month without detectable loss of activity. Frozen aliquots of this solution are stable for at least 6 months when kept at -20˚C. Avoid more than one freeze-thaw cycle.
Species Reactivity
H
Short Description
Biotin - Anti-Human Adipolean
This product belongs to the following categories:
Related Products
Datasheet 1001-W
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