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Primary Antibodies
Adipolean Antibody
Background
Adipolean Antibody
Additional Names
ACDC, ACRP30, Adipocyte C1q and collagen domain containing protein, Adipocyte complement related protein, ADIPOQ, Adipose most abundant gene transcript 1, GBP28, Gelatin binding protein
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Description
Adipose cells produce and secrete numerous physiologically important proteins, such as Lipoprotein Lipase, Leptin, and Adipocyte Complement Related protein of 30 kDa, also known as Acrp30 or Adiponectin. Adiponectin is a circulating protein that is secreted exclusively by differentiated adipocytes. During adipocyte differentiation, Adiponectin mRNA is induced >100 fold. Adiponectin improves the ability of insulin to suppress glucose production, at sub physiological levels, thereby linking adipose tissue to whole body glucose regulation. Adiponectin function appears to be regulated by phosphatidylinositol 3 kinase (PI3K) since Adiponectin secretion is blocked by pharmacologic inhibitors of this kinase. Adiponectin mRNA is significantly reduced in adipose tissue of obese patients with Type 2 diabetes. The structural similarity of Adiponectin to TNF alpha suggests that Adiponectin may play a role in pathogenesis of insulin resistance in Type 2 diabetes. Adiponectin is implicated as a regulator of whole body energy homeostasis.
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Source
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hgAcrp30/Adipolean (human gAcrp30/Adipolean).
Clonality / Clone
This is a polyclonal antibody.
Host
Adipolean antibody was raised in goat.
Please use goat secondary antibodies.
Preparation
A sterile filtered antibody solution (1 mg/mL) was lyophilized from 0.5X PBS pH 7.2.
Application
ELISA:
To detect hgAcrp30/Adipolean by direct ELISA (using 100 μl/well antibody solution) a concentration of at least 0.5μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hgAcrp30/Adipolean.
Western Blot:
To detect hgAcrp30/Adipolean by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hgAcrp30/Adipolean is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Tested Application
E, WB
Storage
Adipolean lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20˚C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/ml. The antibody is stable for at least six weeks at 2-4˚C when reconstituted.
Species Reactivity
H
Short Description
Anti-Human gAcrp30/Adipolean
This product belongs to the following categories:
Related Products
Datasheet 1001-W
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