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Detection Sets
Adipokines: Adiponectin and CTRPs 1-7 Detection Set
Background
Adipose tissue of an organism plays a major role in regulating physiologic and pathologic processes such as metabolism and immunity by producing and secreting a variety of bioactive molecules termed adipokines. One highly conserved family of adipokines is adiponectin/ACRP30 and its structural and functional paralogs, the C1q/tumor necrosis factor-Α-related proteins (CTRPs) 1-7. Unlike the CTRPs, which are expressed in a wide variety of tissues, adiponectin is reported to be expressed exclusively by differentiated adipocytes. These proteins are thought to act mainly on liver and muscle tissue to control glucose and lipid metabolism. Adiponectin is present in high levels in normal human plasma, but is reduced in obese subjects and often in those with increased insulin resistance and type 2 diabetes, suggesting that adiponectin may be a useful pharmacological target in various metabolic diseases. An analysis of the crystal structure of adiponectin revealed a structural and evolutionary link between TNF and C1q-containing proteins, suggesting that these proteins arose from a common ancestral innate immunity gene.
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Description
For images please see PDF data sheet
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Kit Contents
Adiponectin Antibody (CT), Cat. No.. 3551 (50μg)
CTRP1 Antibody (NT), Cat. No.. 3557 (50μg)
CTRP2 Antibody (NT), Cat. No.. 3561 (50μg)
CTRP3 Antibody (IN), Cat. No.. 3565 (50μg)
CTRP4 Antibody (CT), Cat. No.. 3567 (50μg)
CTRP5 Antibody (NT), Cat. No.. 3571 (50μg)
CTRP6 Antibody (CT), Cat. No.. 3575 (50μg)
CTRP7 Antibody (CT), Cat. No.. 3579 (50μg)
Source
Rabbit polyclonal antibodies were raised against peptides corresponding to amino acid sequences from each of the corresponding proteins.
Application
These polyclonal antibodies can be used for detection of the indicated adipokines by Western blot at 1 μg/ml to 2 μg/ml and Immunohistochemistry. Positive control whole cell lysates are available for each of these antibodies. For research use only.
Tested Application
WB, IHC
Blocking Peptide
Adiponectin Peptide, Cat. No.. 3551P
CTRP1 Peptide, Cat. No.. 3557P
CTRP2 Peptide, Cat. No.. 3561P
CTRP3 Peptide, Cat. No.. 3565P
CTRP4 Peptide, Cat. No.. 3567P
CTRP5 Peptide, Cat. No.. 3571P
CTRP6 Peptide, Cat. No.. 3575P
CTRP7 Peptide, Cat. No.. 3579P
Storage
Antibodies are supplied as affinity chromatography purified IgG, in PBS containing 0.02% sodium azide. Antibodies should be stored at -4˚C.
Positive Control
- 293 Lysate (for Adiponectin and CTRP7), Cat. No. 1210
Human Kidney Lysate (for CTRP1), Cat. No. 1305
Caco-2 Lysate (for CTRP2), Cat. No. 1223
Mouse Heart Lysate (for CTRP3), Cat. No. 1401
Rat Brain Lysate (for CTRP4), Cat. No. 1463
Human Brain Lysate (for CTRP5), Cat. No. 1303
HeLa Lysate (for CTRP6), Cat. No. 1201
Species Reactivity
see datasheet
Short Description
Adiponectin, CTRP1, CTRP2, CTRP3, CTRP4, CTRP5, CTRP6, and CTRP7
This product belongs to the following categories:
Related Products
References- Fantuzzi G. Adipose tissue, adipokines, and inflammation. J. Allergy Clin. Immunol. 2005; 115:911-9.
- Tsao T-S, Lodish HF, and Fruebis J. ACRP30, a new hormone controlling fat and glucose metabolism. Euro. J. Pharmacol. 2002; 440:213-21.
- Wong GW, Wang J, Hug C, et al. A family of Acrp30/ adiponectin structural and functional paralogs. Proc. Natl. Acad. Sci. USA 2004; 101:10302-7.
- Lihn AS, Pedersen SB, and Richelsen B. Adiponectin: action, regulation and association to insulin sensitivity. Obes. Rev. 2005; 6:13-21.
Datasheet 0803-W
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