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Primary Antibodies
Adenovirus-9 E4 Orf1 Antibody
Background
The many different serotypes of human adenoviruses (Ad) are divided into six subgroups, of which all Ad subgroup A and B and two subgroup D Ads can elicit tumors in infected rodents. Unlike the Ads from subgroup A and B, the ones from subgroup D, Ad9 and Ad10 elicit estrogen-dependent mammary tumors as opposed to undifferentiated sarcomas. In the case of Ad9, its tumorigenicity is dependent on the product of the open reading frame (ORF) 1 of the early region 4 (E4). The tumorigenic potential of Ad9 E4 Orf1 depends on a carboxyl-terminal PDZ domain-binding motif that mediates interactions with several different membrane-associated cellular proteins such as MUPP1, PATJ, MAGI-1, ZO-2 and Dlg1. It has been suggested that Ad9 E4 Orf1 may have evolved from an ancestral cellular dUTP pyrophosphatase.
Additional Names
Adenovirus-9 E4 Orf1, Ad-9 E4 Orf1
Source
Ad-9 E4 Orf1 antibody was raised against a 16 amino acid peptide near the carboxy terminus of the Ad-9 E4 Orf1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
Adenovirus-9 E4 Orf1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
Ad-9 E4 Orf1 antibody can detect 10ng Ad-9 E4 Orf1 peptide in ELISA at 1 μg/ml.
Tested Application
E
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4749P - Adenovirus-9 E4 Orf1 Peptide
Storage
Adenovirus-9 E4 Orf1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Species Reactivity
V
Protein GI Number
62510674
Protein Accession Number
P89079
Short Description
Open reading frame 1 of the E4 region of Adenovirus 9
This product belongs to the following categories:
Related Products
References- Shenk T. Adenoviridae: the viruses and their replication, p. 2265-2300 in DM Knipe and PM Howley (ed.) Fields virology, 4th ed. Vol. 2. 2001. Lippincott-Raven Publishers, Philadelphia, PA.
- Javier R, Rasha Jr. K, Macdonald GJ, et al. Human adenovirus type 9-induced rat mammary tumors. J. Virol. 1991; 65:3192-202.
- Javier RT. Adenovirus type 9 E4 open reading frame 1 encodes a transforming protein required for the production of mammary tumors in rats. J. Virol. 1994; 68:3917-24.
- Weiss RS, Lee SS, Prasad BV, et al. Human adenovirus early region 4 open reading frame 1 genes encode growth-transforming proteins that may be distantly related to dUTP pyrophosphatase enzymes. 1997; J. Virol. 1997; 71:1857-70.
Datasheet 08-01W
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