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Primary Antibodies
Actin-like 7B Antibody
Background
Actin-like 7B Antibody. This antibody is not expected to cross-react with Actin-like 7A.
Additional Names
ACTL7B, actin-like 7B, MGC151192, MGC151194, Tact1, actin-like 7-beta, t-actin 1
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Description
Left: Western blot analysis of Actin-like 7B in rat testis lysate (35μg protein in RIPA buffer) using Actin-like 7B antibody (0.5μg/mL).
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Source
Actin-like 7B antibody was raised against a synthetic peptide of Actin-like 7B.
Purification
Actin-like 7B antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
Actin-like 7B antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approx 45kDa band observed in rat testis lysates (calculated MW of 45.5kDa according to rat NP_001020588.1 and 45.6kDa according to mouse NP_079547.1). Recommended concentration: 0.5-1.5μg/ml. An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunizing peptide.
Tested Application
E, WB
Buffer
Actin-like 7B antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-199P - Actin-like 7B Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1476 - Rat Testis Tissue Lysate
Species Reactivity
M, R
Protein Accession Number
NP_079547.1
Short Description
Actin-like 7B
This product belongs to the following categories:
Related Products
References- Chadwick BP, Mull J, Helbling LA, Gill S, Leyne M, Robbins CM, Pinkett HW, Makalowska I, Maayan C, Blumenfeld A, Axelrod FB, Brownstein M, Gusella JF, Slaugenhaupt SA. Cloning, mapping, and expression of two novel actin genes, actin-like-7A (ACTL7A) and actin-like-7B (ACTL7B), from the familial dysautonomia candidate region on 9q31. Genomics. 1999 Jun 15;58(3):302-9.
Datasheet 08-01W
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