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Primary Antibodies
Actin Antibody
Background
Actin Antibody.
Additional Names
ACTA2, ACTSA, actin, alpha 2, smooth muscle, aorta, alpha-cardiac actin
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Description
Left: Western blot analysis of Actin in human duodenum lysate (RIPA buffer, 35μg total protein per lane) using Actin antibody (0.3μg/mL).
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Source
Actin antibody was raised against a synthetic peptide of Actin.
Purification
Actin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
Actin antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: antibody detection limit dilution 1:8000. Western Blot: Approx 45kDa band observed in Human Duodenum lysates (calculated MW of 42.0kDa according to NP_001604.1). Recommended concentration: 0.3-1μg/ml.
Tested Application
E, WB, IF
Buffer
Actin antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-198P - Actin Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1364 - Human Duodenum Tissue Lysate
Species Reactivity
H, M, R, D
Protein Accession Number
NP_001604.1
Short Description
Actin
This product belongs to the following categories:
Related Products
References- Reddy S, Ozgur K, Lu M, Chang W, Mohan SR, Kumar CC, Ruley HE. Structure of the human smooth muscle alpha-actin gene. Analysis of a cDNA and 5' upstream region. J Biol Chem. 1990 Jan 25;265(3):1683-7.
Datasheet 09-01W
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