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Recombinant Proteins
Acrp30 Recombinant Protein
Background
The gAcrp30 variant is a naturally occurring globular protein, obtained by proteolytic processing of adiponectin. Adiponectin is produced and secreted exclusively by adipocytes, and is a relatively abundant plasma protein, accounting for up to 0.05% of total serum protein. Like Adiponectin, Acrp30 is capable of decreasing hyperglycemia and reversing insulin resistance. Additionally, gAcrp30 has been shown to be an important factor in promoting fat loss by signaling muscle to absorb and burn Free-Fatty Acids (FFAs). The signaling receptors for adiponectin and gAcrp30 have recently been identified and names AdipoR1 and AdipoR2. AdipoR2 is predominantly expressed in the liver. This naturally occurring variant of human gAcrp30/Adipolean is an 18.1 kDa protein, containing 14 amino acids extra at the N-terminus of human gAcrp30/Adipolean.
Additional Names
apm-1 variant
Location
PGAEGPRGFP GIQGRKGEPG EGAYVYRSAF SVGLETYVTI PNMPIRFTKI FYNQQNHYDG STGKFHCNIP GLYYFAYHIT VYMKDVKVSL FKKDKAMLFT YDQYQENNVD QASGSVLLHL EVGDQVWLQV YGEGERNGLY ADNDNDSTFT GFLLYHDTN
Source
E.coli
Reactivity
n/a
Preparation
Biological activity was determined by a cytotoxicity assay using M1 cells. The expected ED50 for this effect is 0.5 - 1.0 μg/ml.
Tested Application
n/a
Storage
The lyophilized Acrp30 recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted Acrp30 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing Acrp30 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Species Reactivity
n/a
Purity
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Endotoxin level is less than 0.1 ng per μg (1EU/μg).
Short Description
Recombinant Human gAcrp30/Adipolean Variant
This product belongs to the following categories:
Datasheet 0904-W
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