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Primary Antibodies
Acinus Antibody
Background
Chromatin condensation and nuclear fragmentation (CCNF) is the hallmark of apoptosis. CCNF is triggered by the activation of members of caspase family, caspase activated DNase (CAD/DFF40), and several novel proteins including AIF and CIDE . A new inducer of chromatin condensation was recently identified and designated Acinus (for apoptotic chromatin condensation inducer in the nucleus). Acinus is cleaved by caspase-3 and an additional unknown protease generating a small active peptide p17, which causes chromatin condensation in vitro when it is added to purified nuclei. Acinus also induces apoptotic chromatin condensation in cells. Acinus is ubiquitously expressed. Three different spliced forms of Acinus have been identified in human and mouse and designated AcinusL, AcinusS and AcinusS’ (2).
Additional Names
Acinus (CT), Acinus
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Description
Left: Western blot analysis of Acinus in K562 whole cell lysate with Acinus antibody at 0.5 μg/ml.
Below: Immunocytochemistry of Acinus in K562 cells with Acinus antibody at 0.5 μg/ml.
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Other Product Images
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Source
Acinus antibody was raised against a peptide corresponding to amino acids near the carbosy terminus of human AcinusL, which are identical to those of mouse Acinus.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
Acinus antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Murine Acinus (C-Terminus) Peptide (Cat. No. 2217P)
Application
Acinus antibody antibody can be used for detection of Acinus by Western blot at 0.25 – 0.5 μg/ml. An approximate 220 kDa band can be detected.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2217P - Acinus Peptide
Storage
Acinus antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1204 - K562 Cell Lysate
Species Reactivity
H
Protein GI Number
5931959
Protein Accession Number
AAD56724
Short Description
(CT) Inducer of chromatin condensation
This product belongs to the following categories:
Related Products
References- Zamzami N, Kroemer G. Condensed matter in cell death. Nature 1999 ;401:127-8.
- Sahara S, Aoto M, Eguchi Y, Imamoto N, Yoneda Y, Tsujimoto Y. Acinus is a caspase-3-activated protein required for apoptotic chromatin condensation. Nature 1999 401:168-73.
Datasheet 08-02W
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