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Primary Antibodies
Aak1 Antibody
Background
AP2-associated protein kinase 1 (Aak1) is a member of the Ark1/Prk1 subfamily of Ser/Thr protein kinases that are thought to regulate endocytosis by phosphorylating the accessory endocytic components. Aak1 interacts with and phosphorylates the mu2 subunit of the AP-2 complex, which promotes binding of the AP-2 to tyrosine based (Yxxf) internalization motif-containing receptors and subsequent receptor endocytosis. At least two isoforms of Aak1 are known to exist; the longer isoform contains an extended carboxy-terminus that contains an additional clathrin-binding domain. Overexpression of this long isoform or Aak1 depletion by RNA interference impairs transferrin recycling from the early/sorting endosome, suggesting that Aak1 functions at multiple steps of the endosomal pathway by regulating transferrin internalization and its recycling back to the plasma membrane.
Additional Names
Aak1 (NT), AP2-associated protein kinase 1
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Description
Left: Western blot analysis of Aak1 in A-20 lysate with Aak1 antibody at (A) 1 and (B) 2 μg/ml.
Below: Immunocytochemistry of Aak1 in A-20 cells with Aak1 antibody at 5 μg/ml.
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Other Product Images
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Source
Aak1 antibody was raised against a 20 amino acid peptide near the amino terminus of the human Aak1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
Aak1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
Aak1 antibody can be used for detection of Aak1 by Western blot at 1 – 2 μg/ml.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4841P - Aak1 Peptide
Storage
Aak1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1466 - Rat Spleen Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
148277037
Protein Accession Number
NP_055726
Short Description
(NT) AP2-associated protein kinase 1
This product belongs to the following categories:
Related Products
References- Connor SD and Schmid SL. Identification of an adaptor-associated kinase, AAK1, as a regulator of clathrin-mediated endocytosis. J. Cell Biol. 2002; 156:921-9.
- Smythe E and Ayscough KR. The Ark1/Prk1 family of protein kinases. Regulators of endocytosis and the actin skeleton. EMBO Rep. 2003; 4:246-51.
- Ricotta D, Connor SD, Schmid SL, et al. Phosphorylation of the AP2 m2 subunit by AAK1 mediates high affinity binding to membrane protein sorting signals. J. Cell Biol. 2002; 156:791-5.
- Connor SD and Henderson DM. A novel AAK1 splice variant functions at multiple steps of the endocytic pathway. Mol. Biol. Cell 2007; 18:2698-706.
Datasheet 08-01W
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