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Primary Antibodies
AURKB Monoclonal Antibody
Background
AURKB (aurora kinase B, AIK2 or aurora-B), with 344-amino acid protein(about 39kDa),localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers. AURKB is a mitotic protein kinase, which phosphorylates histone H3 and regulates Chromosomal segregation during mitosis and meiosis.It may regulates several stages of mitosis such as centrosome separation, chromosome segregation and cytokinesis.Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. ARK-2 transcripts arepresent at high levels in human thymus and fetal liver.ARK-2 protein levels are maximal during both S and G2/M phases
Additional Names
AURKB
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Description
Western blot analysis using AURKB monoclonal antibody against truncated AURKB recombinant protein and SKN - SH cell lysate.
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Clonality / Clone
This is a monoclonal antibody. Clone 13E8D3.
Isotype
Isotype: IgG1
Host
AURKB monoclonal antibody was raised in mouse.
Please use anti-mouse secondary antibodies.
Immunogen
Ni-NTA purified truncated recombinant AURKB expressed in E. Coli strain BL21 (DE3).
Application
Western Blot:1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Tested Application
E, WB
Storage
AURKB monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1220 - SK-N-SH Cell Lysate
Species Reactivity
H
Formulation
Ascitic fluid containing 0.03% sodium azide.
Purified antibody available at no extra cost.
Protein GI Number
9212
Short Description
aurora kinase B, AIK2 or aurora-B
This product belongs to the following categories:
Related Products
References- Song J et al. Immunity 22:621-31 (2005).
- Kapoor P et al. Mol Cell Biol 25:4934-45 (2005).
Datasheet 0903-W
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