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XW-8124


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REDI-Ladder™ Molecular Weight Marker


Antibody Production


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Primary Antibodies

ATP5B Antibody

Background

FUNCTION: Produces ATP from ADP in the presence of a proton gradient across the membrane. The β chain is the catalytic subunit.

CATALYTIC ACTIVITY: ATP + H2O + H+(In) = ADP + phosphate + H+(Out).

SUBUNIT: F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: α(3), β(3), γ(1), Δ(1), ε(1). CF(0) has three main subunits: a, b and c.

Additional Names

ATP synthase b chain, mitochondrial

ATP5B Antibody

Description

Left: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.

Location

The gene that codes for this protein is at chromosomal location 12q13.13.

Purification

Immunoaffinity Purified

Clonality / Clone

This is a polyclonal antibody.

Host

ATP5B antibody was raised in chicken.

Please use anti-chicken secondary antibodies.

Immunogen

291-539

Application

ATP synthase β chain, mitochondrial antibody can be used for the detection of ATP synthase β chain, mitochondrial by Western Blot.

Tested Application

WB

Buffer

Phosphate-Buffered Saline. No preservatives added.

Storage

ATP5B antibody can be stored at 4˚C for short term (weeks). Long term storage should be at -20˚C. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Species Reactivity

H, M, R

Protein GI Number

4502295

Protein Accession Number

NP_001677.1

Molecular Weight

56.6 kDa (calculated)

Short Description

ATP synthase b chain, mitochondrial,

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