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Primary Antibodies
ATOH8 Antibody
Background
Basic helix-loop-helix (bHLH) transcription factors play important roles in differentiation processes during embryonic development of vertebrates. ATOH8 (MATH6) is a tissue-restricted member of the atonal superfamily of bHLH transcription factors that exhibits 43–57% identity in the bHLH domain with other mammalian atonal paralogs including the NeuroD and Neurogenin factors. In the mouse, ATOH8 has been implicated in the specification and differentiation of neuronal cell lineages in the brain and may also participate in kidney development. Recent studies show that ATOH8 is a novel component of the pancreatic transcriptional network during embryonic development and suggest a potential role as a modulator of the differentiation program initiated by the pro-endocrine factor Neurog3. It is indispensable for early embryonic development, suggesting a more widespread function for this factor in tissue-specific differentiation processes that are dependent on class II bHLH genes.
Additional Names
ATOH8, Helix-loop-helix protein hATH-6, HATH6, Protein atonal homolog 8, MATH6
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Description
Left: Western blot analysis of ATOH8 in A-20 cell lysate with ATOH8 antibody at (A) 1 and (B) 2 μg/ml.
Below: Immunohistochemistry of ATOH8 in mouse brain tissue with ATOH8 antibody at 5 μg/ml.
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Other Product Images
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Source
ATOH8 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human ATOH8.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
ATOH8 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
ATOH8 antibody can be used for detection of ATOH8 by Western blot at 1 – 2 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 5109P - ATOH8 Peptide
Storage
ATOH8 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1288 - A20 Cell Lysate
Species Reactivity
H, M, R
Protein GI Number
226498192
Protein Accession Number
NP_116216
Short Description
Protein atonal homolog 8
This product belongs to the following categories:
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References- Wasserman SM, Mehraban F, Komuves LG, et al. Gene expression profile of human endothelial cells exposed to sustained fluid shear stress. Physiol. Genomics 2002;12:13-23.
- Ledent V and Vervoort M. The basic helix-loop-helix protein family: comparative genomics and phylogenetic analysis. Genome Res. 2001; 11:754–770.
- Inoue C, Bae SK, Takatsuka K, et al. Math6, a bHLH gene expressed in the developing nervous system, regulates neuronal versus glial differentiation. Genes Cells 2001; 6:977–986.
- Ross MD, Martinka S, Mukherjee A, et al. Math6 expression during kidney development and altered expression in a mouse model of glomerulosclerosis. Dev Dyn. 2006; 235:3102–3109.
Datasheet 09-01W
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