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Primary Antibodies
ATG12 Antibody
Background
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG12, another member of the autophagy protein family, forms a conjugate with ATG5; this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells. ATG12 has also been shown to interact with ATG10 in human embryonic kidney cells in the presence of ATG7. At least two isoforms of ATG12 are known to exist.
Additional Names
ATG12 (IN), Autophagy protein 12, Autophagy related protein 12, APG12, APG12L, HAPG12
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Description
Left: Western blot analysis of ATG12 in mouse heart tissue lysate with ATG12 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Source
ATG12 antibody was raised against a 15 amino acid peptide from near the center of human ATG12.
Clonality / Clone
This is a polyclonal antibody.
Host
ATG12 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
ATG12 antibody can be used for the detection of ATG10 by Western blot at 0.5 – 1 μg/ml.
Tested Application
WB
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Storage
ATG12 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1401 - Mouse Heart Tissue Lysate
Species Reactivity
H,M,R
Protein Accession Number
EAW48955
Data Sheet Version
08-02W
Related Products
References
- Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene 2004; 23:2891-906.
- Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.
- Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol. 2000; 150:1507-13.
- Hanada T, Noda NN, Satomi Y, et al. The Atg12-Atg5 conjugate has a novel E3-like activity for protein lipidation in autophagy. J. Biol. Chem. 2007; 282:37298-302.
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