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Primary Antibodies
ATG10 Antibody
Background
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. Another member of the autophagy protein family is ATG10, an E2-like enzyme involved in two ubiquitin-like modifications essential for autophagosome formation: ATG12-ATG5 conjugation and the modification of a soluble form of MAP-LC3, a homolog of yeast Apg8, to a membrane-bound form. ATG10 has also been shown to interact with ATG12 in human embryonic kidney cells in the presence of ATG7. Multiple isoforms of ATG10 are known to exist.
Additional Names
ATG10, Autophagy protein 10, Autophagy related protein 10, APG10L
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Description
Left: Western blot analysis of ATG10 in SK-N-SH cell lysate with ATG10 antibody at (A) 0.5, (B) 1 and (C) 2 μg/ml.
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Source
ATG10 antibody was raised against a 15 amino acid peptide from near the carboxy terminus of human ATG10.
Clonality / Clone
This is a polyclonal antibody.
Host
ATG10 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
ATG10 antibody can be used for the detection of ATG10 by Western blot at 0.5 – 1 μg/ml.
Tested Application
WB
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Storage
ATG10 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1220 - SK-N-SH Cell Lysate
Species Reactivity
H,M,R
Protein Accession Number
EAW95884
Data Sheet Version
08-02W
Related Products
References
- Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene 2004; 23:2891-906.
- Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5.
- Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol. 2000; 150:1507-13.
- Nemoto T, Tanida I, Tanida–Miyake E, et al. The mouse APG10 homologue, an E2-like enzyme for APG12p conjugation, facilitates MAP-LC3 modification. J. Biol. Chem. 2003; 278:39517-26.
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