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Primary Antibodies
ATF6 Antibody
Background
Disruptions of protein folding and maturation in the endoplasmic reticulum (ER) result in the activation of the unfolded protein response (UPR), an integrated cellular signaling pathway that transmits information from the ER lumen to the cytoplasm and nucleus. Activating transcription factor 6 (ATF6) as well as the ER-transmembrane protein kinases IRE1p and PERK are the major transducers of the UPR . ATF6 is an ER transmembrane protein that is normally bound to the ER chaperone GRP78, but upon ER stress is released from GRP78 and proteolytically cleaved to yield a cytosolic fragment which then migrates to the nucleus, and together with the transcription factor XBP-1, activates transcription of UPR-responsive genes (2-4). ATF6 has two isoforms (ATF6Α and ATF6Β); only ATF6Α is recognized by this antibody.
Additional Names
ATF6 (CT), Activating transcription factor 6
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Description
Left: Western blot analysis of ATF6 in MCF7 cell lysate with ATF6 antibody at (A) 0.5 and (B) 1 µg/ml.
Below: Immunocytochemical staining of MCF7 cells using ATF6 antibody at 10 μg/ml.
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Other Product Images
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Source
ATF6 antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human ATF6.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
ATF6 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human ATF6 (C-Terminus) Peptide (Cat. No. 3681P)
Application
ATF6 antibody can be used for the detection of ATF6 by Western blot at 0.5 - 1 μg/ml.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3681P - ATF6 Peptide
Storage
ATF6 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1219 - MCF7 Cell Lysate
Species Reactivity
H, M
Protein GI Number
56786157
Protein Accession Number
NP_031374
Short Description
(CT) An ER signaling protein of the unfolded protein response pathway
This product belongs to the following categories:
Related Products
References- Liu CY and Kaufman RJ. The unfolded protein response. J. Cell Sci. 2003; 1861-2.
- Haze K, Yoshida H, Yanagi H, et al. Mammalian transcription factor ATF6 is synthesized as a transmembrane protein and activated by proteolysis in response to endoplasmic stress. Mol. Cell. Biol. 1999; 10:3787-99.
- Little E, Ramakrishnan M, Roy B, et al. The glucose-regulated proteins (GRP78 and GRP94): functions, gene regulation, and applications. Crit. Rev. Eukaryot. Gene Expr. 1994; 4:1-18.
- Yoshida H, Matsui T, Yamamoto T, et al. XBP1 mRNA is induced by ATF6 and spliced by IRE1p in response to ER stress to produce a highly active transcription factor. Cell 2001; 107:881-91.
Datasheet 08-02W
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