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Primary Antibodies
ASK1 Antibody
Background
Mitogen-activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5 (1-3). ASK1 activated two different subgroups of MAPKK, MKK4 and MKK6, which in turn activated c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death, and a catalytically inactive form of ASK1 inhibited TNF-Α-induced apoptosis. ASK1 is expressed in variety of tissues and cell lines.
Additional Names
ASK1 (C2), Apoptosis signal-regulating kinase, MAPKKK, MEK kinase 5
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Description
Left: Western blot analysis of ASK1 in SW1353 cell lysate with ASK1 antibody at (A) 0.5, (B) 1, and (C) 2 μg/ml.
Below: Immunocytochemistry of ASK1 in A431 cells with ASK1 antibody at 2 μg/ml.
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Other Product Images
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Source
ASK1 antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human ASK1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
ASK1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human ASK1 Peptide (C2) (Cat. No. 3677P)
Application
ASK1 antibody can be used for the detection of ASK1 by Western blot at 1 – 2 μg/ml.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3677P - ASK1 Peptide
Storage
ASK1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1214 - SW1353 Cell Lysate
- Cat. No. 1202 - A431 Cell Lysate
Species Reactivity
H, M
Protein GI Number
66391167
Protein Accession Number
BAA12684
Short Description
(C2) Apoptosis signal-regulating kinase
This product belongs to the following categories:
Related Products
References- Ichijo H, Nishida E, Irie K, et al. Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways. Science 1997; 275:90-4.
- Wang XS, Diener K, Jannuzzi D, et al. Molecular cloning and characterization of a novel protein kinase with a catalytic domain homologous to mitogen-activated protein kinase kinase kinase. J. Biol. Chem. 1996; 271:31607-11.
Datasheet 08-02W
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