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Primary Antibodies
ASK1 Antibody
Background
Mitogen-activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5. ASK1 activated two different subgroups of MAPKK, MKK4 and MKK6, which in turn activated c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death, and a catalytically inactive form of ASK1 inhibited TNF-α-induced apoptosis. ASK1 is expressed in variety of human and mouse tissues.
Additional Names
ASK1 (CT), MAPKKK5
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Description
Left: Western blot analysis of ASK1 in SW1353 whole cell lysate with ASK1 antibody at 1:500 dilution.
Below: Immunocytochemistry of ASK1 in A431 cells with ASK1 antibody at 10 μg/ml.
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Other Product Images
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Source
ASK1 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human ASK1 This sequence is different from that of mouse by last two amino acids.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
ASK1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human ASK1 Peptide (Cat. No. 1151P)
Application
ASK1 antibody can be used for detection of ASK1 by Western blot at 1:500 to 1:1000 dilution. A 155 kDa band can be detected.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 1151P - ASK1 Peptide
Storage
ASK1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1214 - SW1353 Cell Lysate
Species Reactivity
H
Protein GI Number
6685617
Protein Accession Number
Q99683
Short Description
(CT) Apoptosis Signal-regulation Kinase
This product belongs to the following categories:
Related Products
References- Ichijo H, Nishida E, Irie K, ten Dijke P, Saitoh M, Moriguchi T, Takagi M, Matsumoto K, Miyazono K, Gotoh Y. Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways. Science 1997;275:90-4
- Wang XS, Diener K, Jannuzzi D, Trollinger D, Tan TH, Lichenstein H, Zukowski M, Yao Z. Molecular cloning and characterization of a novel protein kinase with a catalytic domain homologous to mitogen-activated protein kinase kinase kinase. J Biol Chem 1996;271:31607-11
- Tobiume K, Inage T, Takeda K, Enomoto S, Miyazono K, Ichijo H. Molecular cloning and characterization of the mouse apoptosis signal-regulating kinase 1. Biochem Biophys Res Commun 1997;239:905-10
Datasheet 08-02W
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