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Primary Antibodies
ASC Antibody
Background
Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD containing cell death regulators include RAIDD, RICK, BCL10, Apaf-1, ARC, caspase-9, and caspase-2. A novel CARD domain containing protein was recently identified in human and mouse and designated ASC and TMS1 (1-3). Ectopic expression of ASC/TMS1 induced apoptosis through activation of caspase-9 and inhibited the survival of human breast cancer cells (3,4). Overexpression of ASC/TMS1 induced DNA fragmentation (4). ASC/TMS1 is expressed in a variety of human and mouse tissues (1, 2)
Additional Names
ASC
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Description
Left: Western blot analysis of ASC in HL60 whole cell lysate in the absence (A) or presence (B) of blocking peptide (2287P) with ASC antibody at 1 μg /ml.
Below: Immunocytochemistry of ASC in HL60 cells with ASC antibody at 5 μg/ml.
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Other Product Images
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Source
ASC antibody was raised against a synthetic peptide corresponding to amino acids 182 to 195 of human ASC .
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
ASC antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human ASC Peptide (Cat. No. 2287P)
Application
ASC antibody can be used for detection of ASC/TMS1 by Western blot at 1 μg/ml.An approximately 25 kDa band can be detected.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2287P - ASC Peptide
Storage
ASC antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1209 - HL60 Cell Lysate
Species Reactivity
H
Protein GI Number
10801602
Protein Accession Number
BAA87339
Short Description
A novel CARD-containing adaptor protein
This product belongs to the following categories:
Related Products
References- Masumoto J, Taniguchi S, Ayukawa K, Sarvotham H, Kishino T, Niikawa N, Hidaka E, Katsuyama T, Higuchi T, Sagara J. ASC, a novel 22-kDa protein, aggregates during apoptosis of human promyelocytic leukemia HL-60 cells. J Biol Chem. 1999;274(48):33835-8.
- Masumoto J, Taniguchi S, Nakayama K, Ayukawa K, Sagara J. Murine Ortholog of ASC, a CARD-Containing Protein, Self-Associates and Exhibits Restricted Distribution in Developing Mouse Embryos. Exp Cell Res. 2001;262(2):128-133.
- Conway KE, McConnell BB, Bowring CE, Donald CD, Warren ST , Vertino PM. TMS1, a novel proapoptotic caspase recruitment domain protein, is a target of methylation-induced gene silencing in human breast cancers. Cancer Res. 2000;60(22):6236-42.
- McConnell BB, Vertino PM. Activation of a caspase-9-mediated apoptotic pathway by subcellular redistribution of the novel caspase recruitment domain protein TMS1. Cancer Res. 2000;60(22):6243-7. (WD0102)
Datasheet 08-02W
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