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Primary Antibodies
ARF-BP1 Antibody
Background
The ARF tumor suppressor is a critical regulator of p53 stability. In addition to p53, ARF1 binds to other proteins such as MDM2 and ARF-BP1, a large protein containing HECT, UBA and WWE motifs. ARF-BP1 directly binds and ubiquitinates p53; this activity is inhibited by ARF, indicating that ARF-BP1 is a critical mediator of the p53-dependent and p53-independent tumor suppressor functions of ARF. ARF-BP1 can also catalyze the polyubiquitination of Mcl-1, an anti-apoptotic Bcl-2 family member involved in DNA damage-induced apoptosis. Elimination of ARF-BP1 expression by RNA interference stabilized Mcl-1 protein, resulting in an attenuation of apoptosis induced by DNA-damage agents.
Additional Names
ARF-BP1, ARF binding protein 1; HECT, UBA and WWE domain containing 1; HUWE1; Mcl-1 ubiquitin ligase E3; Mule
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Description
Left: Western blot analysis of ARF-BP1 in Daudi cell lysate with ARF-BP1 antibody at 1 μg/ml.
Below: Immunocytochemistry of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 5 μg/ml.
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Other Product Images
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Source
ARF-BP1 antibody was raised against a 19 amino acid peptide from near the carboxy terminus of human ARF-BP1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
ARF-BP1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
ARF-BP1 antibody can be used for detection of ARF-BP1 by Western blot at 1 – 2 μg/ml.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4213P - ARF-BP1 Peptide
Storage
ARF-BP1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1224 - Daudi Cell Lysate
Species Reactivity
H, M, R
Protein GI Number
61676188
Protein Accession Number
NP_113584
Short Description
ARF binding protein
This product belongs to the following categories:
Related Products
References- Gallagher SJ, Kefford RF and Rizos H. The ARF tumour suppressor. Int. J. Biochem. Cell Biol. 2006; 38:1637-41.
- Pomerantz J, Schreiber-Agus N, Leigeois NJ, et al. The Ink4a tumor suppressor gene product, p19ARF interacts with MDM2 and neutralizes MDM2’s inhibition of p53. Cell 1998; 92:725-34.
- Chen D, Kon N, Li M, et al. ARF-BP1/Mule is a critical mediator of the ARF tumor suppressor. Cell 2005; 121:1071-83.
- Zhong Q, Gao W, Du F, et al. Mule/ARF-BP1, a BH3-only E3 ubiquitin ligase, catalyzes the polyubiquitination of Mcl-1 and regulates apoptosis. Cell 2005; 121:1085-95.
Datasheet 08-02W
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