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Primary Antibodies
ARC Antibody
Background
Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD domain containing cell death regulators include RAIDD, Apaf-1, caspase-9, and caspase-2. A novel CARD domain containing protein was recently identified and designated ARC for apoptosis repressor with CARD (1). ARC interacts with caspase-2 and –8 and inhibits enzymatic activity of caspase-8. ARC suppresses apoptosis induced by cell death adapters FADD and TRADD and by cell death receptors Fas, TNFR-1 and DR3. The messenger RNA of ARC is primarily expressed in skeletal muscle and cardiac tissue (1).
Additional Names
ARC (CT), ARC
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Description
Left: Western blot analysis of ARC in HeLa (H), KB (K), and A549 (A) whole cell lysates with ARC antibody at 1:1000 dilution.
1st Below: Immunohistochemistry of ARC in human heart tissue at 5 μg/ml.
2nd Below: Immunohistochemistry of ARC in human skeletal muscle with ARC antibody at 5 μg/ml.
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Other Product Images
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Source
ARC antibody was raised against a peptide corresponding to amino acids 191 to 208 of human origin.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
ARC antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human ARC (C-Terminus) Peptide (Cat. No. 2081P)
Application
ARC antibody can be used for detection of ARC by Western blot at 1:1000 dilution. An approximately 25 kDa band can be detected.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2081P - ARC Peptide
Storage
ARC antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1201 - HeLa Cell Lysate
Species Reactivity
H
Protein GI Number
4505419
Protein Accession Number
NP_003937
Short Description
(CT) Apoptosis Repressor with CARD domain
This product belongs to the following categories:
Related Products
References- Koseki T, Inohara N, Chen S, Nunez G. ARC, an inhibitor of apoptosis expressed in skeletal muscle and heart that interacts selectively with caspases. Proc Natl Acad Sci U S A 1998;95:5156-60 (RD1299)
Datasheet 08-02W
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