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Primary Antibodies
APPL1 Antibody
Background
APPL1 Antibody.
Additional Names
APPL1, DIP13alpha, adaptor protein containing pH domain, PTB domain and leucine zipper motif, signaling adaptor protein DIP13alpha
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Description
Left: Western blot analysis of APPL1 in 1) rat aortic smooth muscle cell lysate 2) after infection with human APPL1 adenovirus for 48hrs using APPL1 antibody (0.5μg/mL).
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Source
APPL1 antibody was raised against a synthetic peptide of APPL1.
Purification
APPL1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
APPL1 antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approx 80kDa band observed in rat Aortic Smooth muscle Cells (lane 1), intensity increased after infection with human APPL1 adenovirus for 48h (lane 2). Calculated MW of 79.7kDa according to NP_036228.1. The blot was co-labeled with Tubulin-specific antibodies as a loading control. Recommended concentration: 0.3-1μg/ml.
Tested Application
E, WB
Buffer
APPL1 antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-271P - APPL1 Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Species Reactivity
H, M, R, D, B
Protein Accession Number
NP_036228.1
Short Description
APPL1
This product belongs to the following categories:
Related Products
References- Mao X, Kikani CK, Riojas RA, Langlais P, Wang L, Ramos FJ, Fang Q, Christ-Roberts CY, Hong JY, Kim RY, Liu F, Dong LQ. APPL1 binds to adiponectin receptors and mediates adiponectin signalling and function. Nat Cell Biol. 2006 May;8(5):516-23. Epub 2006 Apr 16. Erratum in: Nat Cell Biol. 2006 Jun;8(6):642.
Datasheet 08-01W
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