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Primary Antibodies
APPD Antibody
Background
APPD (Apoptosis-inducing protein D; phafin 1) is predicted through gene annotation and contains pleckstrin homology domain. It may binds to two Zn++ ions through its FYVE zinc finger domain. Biological function of this protein has not been clearly mapped yet.
Additional Names
APPD
Purification
Antibody is purified by Protein A/G affinity chromatography method.
Clonality / Clone
This is a polyclonal antibody.
Host
APPD antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human APPD.
Application
APPD antibody can be used for detection of APPD by ELISA at 1:120. APPD antibody can be used for detection of APPD by western blot at 1 μg/ml, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Tested Application
E, WB
Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 100 μl of distilled water. Final antibody concentration is 1 mg/ml.
Storage
For longer periods of storage, store APPD antibody at -20˚C. Avoid repeat freeze-thaw cycles.
Species Reactivity
H
Protein Accession Number
NP_077286
Molecular Weight
31 kDa
Short Description
APPD (apoptosis-inducing protein D)
This product belongs to the following categories:
References- Strausberg, R.L., et al (2002) Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903..
Datasheet 10-01W
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