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Primary Antibodies
APPD Antibody
Background
APPD (Apoptosis-inducing protein D; phafin 1) is predicted through gene annotation and contains pleckstrin homology domain. It may binds to two Zn++ ions through its FYVE zinc finger domain. Biological function of this protein has not been clearly mapped yet.
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Description
Left: Western blot analysis of APPD expression in HepG2(A), Raji (B),293T(C) cell lysates using anti-APPD.
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Purification
Rabbit IgG is purified by Protein A/G affinity chromatography method.
Clonality / Clone
This is a polyclonal antibody.
Host
APPD antibody was raised in rabbit.
Please use anti-rabbit IgG secondary antibodies.
Immunogen
APPD antibody was produced in rabbits immunized with a synthetic peptide corresponding to a region of human PLEKHF1.
Application
APPD antibody can be used for Western blot at a suggested dilution of 1 μg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody. The protein size is 279 amino acids.
Tested Application
WB
Buffer
Antibody is supplied in PBS buffer with 2% sucrose. Product may ship lyophilized. To reconstitute add 100μl of distilled water making the final concentration 1 mg/ml.
Storage
APPD antibody is supplied in PBS buffer containing 0.02% sodium azide. For longer periods of storage, store at -20ºC. Avoid repeat freeze-thaw cycle.
Positive Control
- Cat. No. 1211 - HepG2 Cell Lysate
- Cat. No. 1207 - Raji Cell Lysate
- Cat. No. 1210 - 293 Cell Lysate
Species Reactivity
H
Protein Accession Number
NP_077286
Molecular Weight
30 kDa
Short Description
Anti-APPD
This product belongs to the following categories:
Related Products
References- Strausberg, R.L., et al (2002) Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903.
Datasheet 0802-W
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