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Recombinant Proteins
APP Recombinant Protein
Background
Accumulation of the amyloid-beta peptide (Abeta) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer’s disease. The beta?amyloid protein precursor (APP) is cleaved by one of two beta?secretases (BACE and BACE2), producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for gamma?secretase to generate the 4 kDa amyloid-beta peptide (Abeta), which is deposited in the Alzheimer’s disease patients’ brains. Recently, Death Receptor 6 (DR6) was found to interact with an amino-terminal fragment of the Beta-amyloid protein (N-APP) in neurons, activating a caspase 6-dependent apoptotic event leading to axonal degeneration and pruning during development, suggesting that these two proteins are involved in neural development and may possibly play a role in Alzheimer’s disease. The DR6 extracellular domain recombinant protein will bind to this N-APP protein fragment in vitro.
Additional Names
N-terminal APP
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Description
Left: SDS-PAGE analysis of recombinant N-APP on Coomassie Blue-stained 10% SDS-PAGE gel.
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Source
E. coli
Application
This recombinant protein can be used for WB, ELISA, MS and neutralization assays.
Tested Application
E, WB, MS
Buffer
1X PBS containing 10% glycerol
Storage
Store at -70˚C. As with any protein, exposing IL-16 recombinant protein to repeated freeze/thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Species Reactivity
n/a
Protein GI Number
112927
Protein Accession Number
P05067
Domain Information
aa 18 – 286
Molecular Weight
36 kDa (Calculated)
Fusion Partner
C-terminal His-tag
Purity
~95%
Short Description
E. coli expressed Human APP N-terminal Domain
This product belongs to the following categories:
References- Ponte P, Gonzalez-DeWhitt P, Schilling J, et al. A new A4 amyloid mRNA contains a domain homologous to serine proteinase inhibitors. Nature 1988; 331:525-77.
- Selkoe D.J. Cell biology of the amyloid beta-protein precursor and the mechanism of Alzheimer's disease. Annu Rev Cell Biol 1994; 10:373-403.
- Nikolaev A, McLaughlin T, O’Leary DD, et al. APP binds DR6 to trigger axon pruning and neuron death via distinct caspases. Nature 2009; 457:981-9.
Datasheet 09-01W
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