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Primary Antibodies
APP Monoclonal Antibody
Background
Amyloid precursor protein(APP) ,with 32-amino acid protein (about 35 kDa), is part of a super-family of transmembrane and secreted proteins. It appears to have a number of roles, including regulation of haemostasis and mediation of neuroprotection. APP undergoes alternative splicing, resulting in several isoforms . Proteolytic cleavage of amyloid precursor protein leads to the formation of the 4 kDa amyloid beta/A4 protein, which is present in human platelets. APP is also involved in the formation of neurofibrillary tangles and plaques that characterize the senile plaques of Alzheimer patients.
Additional Names
APP
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Description
Left: Western blot analysis using APP monoclonal antibody against truncated TGF APP recombinant protein
Below: Immunohistochemical analysis of paraffin - embedded human Alzheimer brain tissue showing cytoplasmic localization using APP antibody with DAB staining.
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Other Product Images
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Clonality / Clone
This is a monoclonal antibody. Clone 1B11F3.
Isotype
IgG2a
Host
APP monoclonal antibody was raised in mouse.
Please use anti-mouse secondary antibodies.
Immunogen
Ni-NTA purified truncated recombinant APP expressed in E. Coli strain BL21 (DE3).
Application
Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000
IHC(F):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Tested Application
WB, IHC
Storage
APP monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Species Reactivity
H
Formulation
Ascitic fluid containing 0.03% sodium azide.
Purified antibody available at no extra cost.
Protein GI Number
351
Short Description
Amyloid precursor protein(APP)
This product belongs to the following categories:
References- Yuzhi Chen, Wenyun Liu, Donna L, et al.J. Cell Biol., Oct 2003; 163: 27.
- Robert Spoelgen, Christine A. F. von Arnim, et al. J. Neurosci., Jan 2006; 26: 418 – 428.
Datasheet 0903-W
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