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Primary Antibodies
APP Antibody
Background
Accumulation of the amyloid-beta peptide (Abeta) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer’s disease. The beta?amyloid protein precursor (APP) is cleaved by one of two beta?secretases (BACE and BACE2), producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for gamma?secretase to generate the 4 kDa amyloid-beta peptide (Abeta), which is deposited in the Alzheimer’s disease patients’ brains. Recently, Death Receptor 6 (DR6) was found to interact with an amino-terminal fragment of the Beta-amyloid protein (N-APP) in neurons, activating a caspase 6-dependent apoptotic event leading to axonal degeneration and pruning during development, suggesting that these two proteins are involved in neural development and may possibly play a role in Alzheimer’s disease.
Additional Names
APP (NT2), Amyloid Precursor Protein, amyloid-beta precursor protein
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Description
Left: Western blot analysis of APP in rat brain tissue lysate with APP antibody at (A) 1 and (B) 2 μg/ml.
Below: Immunohistochemistry of APP in mouse brain tissue with APP antibody at 2.5 μg/ml.
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Other Product Images
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Source
APP antibody was raised against an 18 amino acid peptide near the amino terminus of human APP.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
APP antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
APP antibody can be used for detection of APP by Western blot at 1 – 2 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 5143P - APP Peptide
Storage
APP antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1463 - Rat Brain Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
112927
Protein Accession Number
P05067
Short Description
(NT2) Amyloid precursor protein
This product belongs to the following categories:
References- Ponte P, Gonzalez-DeWhitt P, Schilling J, et al. A new A4 amyloid mRNA contains a domain homologous to serine proteinase inhibitors. Nature 1988; 331:525-77.
- Selkoe DJ. Cell biology of the amyloid beta-protein precursor and the mechanism of Alzheimer's disease. Annu Rev Cell Biol 1994; 10:373-403.
- Nikolaev A, McLaughlin T, O’Leary DD, et al. APP binds DR6 to trigger axon pruning and neuron death via distinct caspases. Nature 2009; 457:981-9.
Datasheet 09-01W
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