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Primary Antibodies
APP Antibody
Background
Accumulation of the amyloid-β peptide (αβ) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. The b-amyloid protein precursor (APP) is cleaved by β-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for g-secretase to generate the 4 kDa amyloid-β peptide (αβ), which is deposited in the brains of all suffers of Alzheimer’s disease.
Additional Names
APP (CT), APP
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Description
Left: Western blot analysis of APP in mouse (M) and rat (R) brain tissue lysates with APP antibody at 2 μg/ml.
Below: Immunohistochemical staining of rat brain using APP antibody at 2 μg/ml.
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Other Product Images
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Source
APP antibody was raised against a peptide corresponding to amino acids 737 to 751 of human amyloid A4 protein precursor (APP) or 85 to 99 of the C99 fragment generated by b-secretase cleavage. The peptide sequences are identical to those of monkey, mouse, rat, chicken, and a variety of other species .
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
APP antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human APP (C-Terminus) Peptide (Cat. No. 2136P)
Application
APP antibody can be used for detection of APP and the C99 fragment by Western blot at 1 – 2 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2136P - APP Peptide
Storage
APP antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1403 - Mouse Brain Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
28721
Protein Accession Number
CAA30050
Short Description
(CT) Amyloid protein precursor
This product belongs to the following categories:
Related Products
References- Ponte P, Gonzalez-DeWhitt P, Schilling J, Miller J, Hsu D, Greenberg B, Davis K, Wallace W, Lieberburg I, Fuller F. A new A4 amyloid mRNA contains a domain homologous to serine proteinase inhibitors. Nature 1988;331:525-7
- Selkoe D.J. Cell biology of the amyloid β-protein precursor and the mechanism of Alzheimer's disease.Annu Rev Cell Biol 1994;10:373-403 (RD1299)
Datasheet 08-02W
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