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Primary Antibodies
APOM Antibody
Background
APOM Antibody.
Additional Names
APOM, apolipoprotein M, G3a, HSPC336, MGC22400, NG20, NG20-like protein, G3a, NG20
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Description
Left: Western blot analysis of APOM in paraffin embedded human liver using APOM antibody (5μg/mL).
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Source
APOM antibody was raised against a synthetic peptide of APOM.
Purification
APOM antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
APOM antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: Antibody detection limit dilution 1:8,000. Western Blot: Preliminary experiments gave an approx 50kDa band in human kidney and liver lysates after 0.2μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 21.3kDa according to NP_061974.2. We do note that ApoM is a glycoprotein which makes a higher size band to be expected. The 50kDa band was successfully blocked by incubation with the immunizing peptide.
Tested Application
E, IHC
Buffer
APOM antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-270P - APOM Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1304 - Human Liver Tissue Lysate
Species Reactivity
H, D
Protein Accession Number
NP_061974.2
Short Description
APOM
This product belongs to the following categories:
Related Products
References- Xu N, Nilsson-Ehle P, Ahren B. Correlation of apolipoprotein M with leptin and cholesterol in normal and obese subjects. J Nutr Biochem. 2004 Oct;15(10):579-82.
Datasheet 08-01W
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